Skip to main content
. Author manuscript; available in PMC: 2019 Jul 7.
Published in final edited form as: Mucosal Immunol. 2019 Jan 7;12(2):445–456. doi: 10.1038/s41385-018-0117-1

Figure 8. SCF mediated ILC2 activation in vitro.

Figure 8.

A) ILC2 were sorted from a Lin− CD45+ enriched cells population from the lung of allergic mice by flow cytometry, B) Identity of the sorted cells was analyzed by qPCR to detected transcription factors GATA3, RoRc and T-bet. C) Sorted cells were cultured in vitro for 16 h in untreated media (RPMI) with or without murine SCF (rSCF) and qPCR was performed to detect GATA3 D) and ID2 E) Sorted cells were cultured in vitro for 24 h alone or in presences of recombinant murine SCF (rSCF), and the cytokine expression was analyzed by qPCR, C) Expression of cytokine receptors by qPCR in SCF stimulated ILC2, F) ILC2 cultured with SCF and/or IL-25 (rIL-25) were analyzed by qPCR for expression of cytokines. Data represent mean ± SE of 4 replicates/experiment. All data are representative of three independent experiments. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.