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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Circ Res. 2019 Feb 15;124(4):e6–e19. doi: 10.1161/CIRCRESAHA.118.313028

Figure 2. Myeloid-specific deletion of epsins decreases immune and inflammatory cell content in the aorta, increases smooth muscle cell content, and decreases necrotic core content within the atheroma.

Figure 2.

A. Aortas from ApoE−/−(n=5) and ApoE−/−/LysM-DKO (n=5) fed Western diet (WD) were isolated, digested, cells isolated, labeled with CD45 (hematopoietic cells), CD11b (granulocytes, monocytes, macrophages, dendritic cells, NK cells), F4/80 (macrophage; F4/80+CD11b+ defined as macrophage), CD19 (B cells), TCRβ (T cells), Ly6C (monocytes), Ly6G (neutrophils defined as Ly6C+;Ly6G+), and analyzed via flow cytometry. *ApoE−/−/LysM-DKO group vs. ApoE−/− group, P<0.01. Aortic root sections from ApoE−/− (n=8) and ApoE−/−/LysM-DKO (n=10) fed WD for 20 weeks (B) and 25 weeks (C) were stained with CD68 (macrophages, green) and α-Smooth Muscle Actin (αSMA) (red, smooth muscle cells) in B and H&E in C. Scale bar=200μM. *ApoE−/−/LysM-DKO group vs. ApoE−/− group, P<0.01.