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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: Mucosal Immunol. 2018 Nov 29;12(2):518–530. doi: 10.1038/s41385-018-0106-4

Figure 1. Expression of CCL2 (MCP-1) in the lung is increased following H1N1 infection or H1N1 / MRSA dual infection.

Figure 1.

(A) Bronchoalveolar lavage (BAL) was performed on uninfected, H1N1 or H1N1 / MRSA dual infected WT mice (6 days after H1N1 infection, 24 hours following MRSA infection). CCL2 expression was measured via qRT-PCR from isolated BAL cells. (B) Leukocytes were harvested from collagenase digested lung tissue 24 hours after MRSA infection (6 days after H1N1 infection). CCL2 expression was measured via qRT-PCR. (C) Whole lung homogenates were prepared 24 hours after MRSA infection (6 days following H1N1 infection). CCL2 was quantified by ELISA. Statistical significance was calculated by Kruskal Wallis non-parametric test, * p < 0.05, ** p < 0.01 versus uninfected. n = 3 mice per group.