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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: Mucosal Immunol. 2018 Nov 29;12(2):518–530. doi: 10.1038/s41385-018-0106-4

Figure 6. Isolated Alveolar Epithelial Cells express IL-17 receptor and secrete CXCL1 in response to both IL-17 stimulation and H1N1 infection.

Figure 6.

(A) Type II alveolar epithelial cells were isolated from either WT or CCR2−/− mice and infected with H1N1 (MOI = 0.01) or left uninfected for 48 h. Total RNA was extracted and analyzed for the expression of IL-17Rα. (B) Type II alveolar epithelial cells were treated either with recombinant IL-17 (10 ng / mL), H1N1 (MOI = 0.01), or a combination of IL-17 and H1N1. After 48 h of treatment supernatants were analyzed for CXCL1 by ELISA. Statistical significance was determined using ANOVA (A), or Kruskal-Wallis non-parametric test (B) * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001, n = 3 – 5 biological replicates per group.