Table 1.

Digestive Enzymes in Solid Tissue Disaggregation

ENZYME	PURPOSE
Dispase	-Breaks down extracellular matrix
	-Detaches cell colonies
	-Cleaves attachments between cells and extracellular matrix
Collagenase	-Breaks down extracellular matrix
	-Breaks peptide bonds present in collagen
Hyaluronidase	-Breaks down extracellular matrix
	-Cleaves glycosidic bonds in hyaluronan
Papain	-Degrades proteins which make up tight junctions between cells
DNase-I	-Degrades free-DNA
	-Prevents cell aggregation
Accutase	-Proteolytic, collagenolytic, and DNase activity
TrypLE	-Cleaves cell-cell junctions
	-Does not alter antigen expression as trypsin would