Fig. 2.

MA-ARDS/ALI is primarily associated with CXCR3+ and Tbet+ T cells. a Expression of CCR4 (left panels) and CXCR3 (right panels) on CD4⁺ T cells (top panels) or CD8⁺ T cells (bottom panels) from uninfected and PbN-infected C57BL/6 mice. Graphs indicating MFI of the CCR4 and CXCR3 expression by CD8+ and CD4+ are shown in the right. b Total number of CD8⁺ (top panel) and CD4⁺ (bottom panel) T cells in the lungs of WT and CCR4^−/− mice infected with PbN. c Intracellular staining of the transcription factors Tbet, GATA-3, RORγt, and FoxP3 in CD4⁺ T cells from uninfected controls (top panels) or PbN-infected (bottom panels) WT (left panels) and CCR4^−/− mice (right panels). d Total number of Tbet⁺ (left top), GATA-3⁺ (right top), RORγt⁺ and FoxP3⁺ (right bottom) CD4⁺ T and CD8⁺ T cells in the lungs of uninfected controls (white bars) and PbN-infected (black bars) WT and CCR4^−/− mice. Data (a–d) are representative of at least two independent experiments (n = 4–6 mice per group)