Fig. 3.

Emergence of TNF-α and iNOS producing dendritic cells (Tip-DC) in the lungs from PbN infected mice. a Dot plots indicating kinetics of F4/80⁺CD11b⁺Ly6c⁺ cells in the lungs of PbN-infected WT mice. b Characterization of the F4/80⁺CD11b⁺Ly6c⁺ cells from uninfected and infected WT mice at 9 days post-infection by flow cytometry shows that they are CD68^-CD115⁻CD135⁻DCsign⁺CD11c⁺CD80⁺CD86⁺MHCII⁺. c Intracellular staining of TNFα and NOS2 expression by MO-DC harvested from lungs of PbN-infected C57BL/6 mice at 9 days post infection. Dot plots with frequency (left) and graph with total number (right) of Tip-DCs, alveolar macrophages (Alveolar MO) and neutrophils. Data (a–c) are representative of at least two independent experiments (n = 4–6 mice per group)