Fig. 4.

Emergence of Tip-DCs in the lungs of PbN-infected mice is mediated by CCR4. a Expression of CCR4 ligands in the lungs of uninfected and PbN-infected WT mice was evaluated by qPCR. b Quantification of CCL2 by ELISA from homogenate originated from lungs of C57BL/6 mice. c Histogram showing CCR2 and CCR4 expression by monocyte-derived cells (F4/80⁺CD11b⁺) harvested from uninfected and infected C57BL/6 mice (top panel). Graphs indicating MFI of the CCR2 and CCR4 expression by F4/80⁺CD11b⁺ that refers to the representative histograms (top panel). d Dot plots showing the frequency of F4/80⁺CD11b⁺Ly6c⁻ and F4/80⁺CD11b⁺Ly6c⁺ monocytes in the lungs of control and PbN-infected WT, CCR2^−/−, and CCR4^−/− mice. e Total number of F4/80⁺CD11b⁺Ly6c⁺ cells found in the lungs of uninfected controls and PbN-infected WT, CCR2^−/−, and CCR4^−/− mice. f Survival curve of CCR2^−/−, CCR4^−/−, and WT mice after challenge with PbN. Data (a–e) are representative of at least two independent experiments (n = 4–6 mice per group)