Fig. 6.

NOS2^−/− mice are resistant to PbN-induced MA-ARDS/ALI. a Lungs from uninfected (top quadrants) and PbN-infected (bottom quadrants) WT and NOS2^−/− mice. b Survival curves of WT and NOS2^-/- mice infected with PbN. c Dot plot showing the frequency of F4/80⁺CD11b⁺Ly6c⁺ cells in WT and NOS2^−/− mice. Histogram showing the DCsign expression (right, top panel) and MFI graph (right, bottom panel) of F4/80⁺CD11b⁺Ly6c⁺ cells from lungs of control and PbN-infected WT and NOS2^−/− mice. d Gate strategy (dot plots) to quantify the frequency of NOS2⁺ expressing cells. Graph indicates the frequency of Tip-DCs (F4/80⁺CD11b⁺CD11c⁺TNF-α⁺NOS2⁺) compared to non-Tip-DC leukocyte populations that express NOS2 in the lungs of WT infected mice. Data (a–d) are representative of two independent experiments (n = 4–5 mice per group).