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. Author manuscript; available in PMC: 2019 Jul 7.
Published in final edited form as: Mucosal Immunol. 2019 Jan 7;12(2):545–554. doi: 10.1038/s41385-018-0115-3

Figure 3. Neutrophil depletion in mice from the first week p.i. (post-infection) reduces lung damage and lung inflammatory cytokines levels without impacting viral clearance.

Figure 3.

Aged and young mice were infected with influenza virus and depleted of neutrophils or treated with isotype control at day 6 p.i and then intermittently until day 12 p.i. At day 10 p.i., the BAL was obtained from and LDH (a), protein (b), IL-1β (c), TNF-α (d) measured via ELISA * P < 0.05 ** P = 0.01 (Mann-Whitney test). Data are representative of one of two independent experiments, which yielded similar results. Data are expressed as mean ± SEM eYoung and aged mice were infected with influenza and depleted of neutrophils or treated with isotype control at day 6 p.i and then intermittently until day 10 p.i. At day 10 p.i, lungs were obtained and viral load measured by plaque assay. There were no significant differences in viral load between control and neutrophil depleted groups. P = 0.49 (young Rat IgG vs Anti-Ly6G(Mann-Whitney test) and P = 0.47 (aged Rat IgG vs Anti-Ly6G) (Mann-Whitney test). Data are representative of two independent experiments.