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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Am J Transplant. 2018 Sep 17;19(3):633–645. doi: 10.1111/ajt.15072

Figure 2.

Figure 2.

Absence of myeloid cell expressed C5ar1 augments donor-reactive T cell immune responses in MR1-treated allograft recipients. A. Donor-reactive IFNγ ELISPOTs from C5ar1fl/fl and C5ar1fl/flxLysM-Cre recipients of BALB/c hearts 14 days post-transplant. n=9/group. Frequencies of donor-reactive IFNγ-producers in spleens of untreated C5ar1fl/fl and C5ar1fl/flxLysM-Cre recipients of BALB/c hearts at rejection (day 7) were ~1300/5×105 spleen cells and were not different between groups (not shown). B-C. Representative flow cytometry plots (B) and total numbers per spleen of donor-reactive IFNγ-producing CD8+ T cells (C) from C5ar1fl/fl and C5ar1fl/flxLysM-Cre recipients of BALB/c hearts 14 days post-transplant, n=12/group. D. Serum donor-reactive alloantibodies in C5ar1fl/fl and C5ar1fl/flxLysM-Cre recipients of BALB/c hearts 14 days post-transplant, n=3/group E-F. Total numbers of Foxp3+CD4+ T cells per spleen (E) and calculated ratios of CD4+Foxp3+ cells/donor reactive IFNγ-producers per mouse (TREG:TEFF ratios), n=12/group (F). * p<0.05