Figure 7.

Graft infiltrating C5ar1^fl/fl and C5ar1^fl/flxLysM-Cre Ly6C^lo myeloid cells exhibit equivalent suppressive capacities and rates of apoptosis. A. Heatmap depicting expression levels for M_REG-produced genes involved in suppressive function (see Figure 5 for details, n=3–4/group). B. Representative flow cytometry plots of unstimulated or anti-CD3 stimulated WT T cells ± flow sorted CD11b⁺Ly6C^loLy6G^- myeloid cells (200,000 T cells, 50,000 CD11c⁺ APCs and 50,000 myeloid cells) isolated from allografts on day 6 of MR1-treated C5ar1^fl/fl (controls) or C5ar1^fl/flxLySM-Cre recipients, illustrating suppressive ability of myeloid cells from both genotypes. Representative of results from 2 independent experiments. C. Heatmap depicting expression levels genes involved in cell death/survival (see Figure 5 for details, n=3–4/group). D-E. Representative flow cytometry plot (D) and quantified results (E) depicting frequency of apoptotic (AnnexinV⁺) CD11b+ Ly6C^lo graft-infiltrating cells. *p<0.05, ns=not significant.