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. 2019 Feb 8;7:54. doi: 10.3389/fchem.2019.00054

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Copyright © 2019 Zhang, Liu, Li, Li and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence microscopy imaging of hERG transfected HEK293 cells incubated with 5 μM probe L1, 5 μM probe L2, 1 μM probe L3, 5 μM probe L4 (A1, bright field; A2, GFP channel), respectively. The imaging of inhibition of the hERG channels was accomplished by incubating astemizole (50, 50, 10, 50 μM) with probe L1 (5 μM), L2 (5 μM), L3 (1 μM), and L4 (5 μM) (A3, bright field; A4, GFP channel). All cells were incubated with each probe at 37°C for 10 min and washed immediately. The background was adjusted by ImageJ software. Imaging was performed using a Zeiss Axio Observer A1 microscope with a 63 × objective lens. .

Inline graphic — Fluorescence microscopy imaging of hERG transfected HEK293 cells incubated with 5 μM probe L1, 5 μM probe L2, 1 μM probe L3, 5 μM probe L4 (A1, bright field; A2, GFP channel), respectively. The imaging of inhibition of the hERG channels was accomplished by incubating astemizole (50, 50, 10, 50 μM) with probe L1 (5 μM), L2 (5 μM), L3 (1 μM), and L4 (5 μM) (A3, bright field; A4, GFP channel). All cells were incubated with each probe at 37°C for 10 min and washed immediately. The background was adjusted by ImageJ software. Imaging was performed using a Zeiss Axio Observer A1 microscope with a 63 × objective lens. .