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. 2019 Feb 8;9:31. doi: 10.3389/fonc.2019.00031

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Copyright © 2019 Himes, Zhang and Daniels.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cannula-guided convection enhanced delivery in the rat pons (Daniels Laboratory—Mayo Clinic). (A) Infusion pump is attached to the cannula installed on rat brain where the infusate was delivered at a constant rate over time. (B) Photograph of ink solution injected at 8 mm of depth with a Hamilton syringe through the cannula validating V_d. (C) Coronal section of athymic nude rat brainstem with DIPG patient derived xenograft showing representative images of low magnification scan of H&E and high magnification scan of H3K27M and H3K27me3 immunohistochemical (IHC) staining.