Figure 2.

Sequestering BDNF induces neuronal death after SE in neurons-astrocytes co-cultures. (A) SE was induced in co-cultures for 3 h (“No Mg²⁺ buffer”) followed by growth media restitution. Control cultures received a buffer with MgCl₂. After 12 h, cells were immunostained for NeuN and βIII-tubulin, plus DAPI. (B) Representative micrographs of NeuN (green), βIII-tubulin (red) and DAPI (blue) staining in control, control + TrkB-Fc (Fc), or 12 h post-SE 12 h post-SE + TrkB-Fc hippocampal neurons. Scale bar = 50 μm. (C) Quantification of NeuN-positive cells in co-cultures. Data are expressed as mean ± SEM (n = 3 independent experiments with three pseudo-replicates per experiment). Asterisk indicates p < 0.05 compared to control cultures by one-way ANOVA followed by Tukey’s post hoc analysis.