Figure 3.

BDNF prevents proBDNF-induced neuronal death. (A) Representative micrographs of hippocampal neurons stained with NeuN, βIII-tubulin and DAPI. The following conditions were studied: untreated control, cells treated with 100 ng/ml BDNF, with 100 ng/ml proBDNF, or with BDNF + proBDNF. Scale bar = 50 μm. (B) Quantification of NeuN-positive cells. Data are expressed as mean ± SEM (n = 3 independent experiments with three pseudo-replicates per experiment). Asterisk indicates p = 0.024 compared to control by one-way ANOVA followed by Tukey’s post hoc analysis.