FIGURE 2.

Identification of cis-regulatory elements involved in HBV 3.5-kb RNA splicing in cell-type dependent and -independent manners. (A) A schematic representation of constructs and PCR primers used in the intron-swapping experiment is indicated. (B) HBV WT and HBV/int-SV40 were expressed in HepG2 and HEK293 cells. PCR products were analyzed by agarose gel electrophoresis (left). Schematic of unspliced and spliced forms detected is shown (right). (C) SV40-L WT and SV40/int-HBV were expressed in the cells and PCR products were analyzed as shown in (B).