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. 2019 Feb 8;10:63. doi: 10.3389/fgene.2019.00063

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Copyright © 2019 Zotova, Kurishbayev, Jatayev, Goncharov, Shamambayeva, Kashapov, Nuralov, Otemissova, Sereda, Shvidchenko, Lopato, Schramm, Jenkins, Soole, Langridge and Shavrukov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Allele discrimination in eight wheat cultivars (A) and in the segregating population 18-6 (B) using the Amplifluor-like SNP marker KATU-W62. X- and Y-axes show relative amplification units, ΔR_n, for FAM and VIC fluorescence signals, respectively. Red dots represent homozygote (aa) genotypes with allele 1 (FAM) associated with the high yielding cultivars, blue dots represent homozygote (bb) genotypes for allele 2 (VIC), and green dots represent heterozygote (ab) or mixed genotypes identified with automatic SNP calling. The black squares show the no template control (NTC) using water instead of template DNA.