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. 2019 Feb 14;9:2050. doi: 10.1038/s41598-018-35535-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Curve fitting for K_d determination. (A) Decreased emission of Em₄₇₅ was calculated by subtracting the emission intensity of CyPet-SUMO1 in the presence of YPet-Ubc9 from the emission in the absence of YPet-Ubc9. The value of decrease was then fitted with total YPet-Ubc9 concentration to derive the value of K_d according the formula Y = n/2 (A + X + K_d − √((A + X + K_d)^2−4AX)) (see text for details). (B) Linearity of maximum quenching signal of CyPet-SUMO1 by Ypet-Ubc9 at different concentrations was determined.