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. 2019 Feb 14;10:746. doi: 10.1038/s41467-019-08524-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — PLCβ2 interacts with TAK1. a–c IB or immunoprecipitates (IP) of lysates from HEK293T cells transfected with various plasmids as indicated. d In vitro GST precipitation assay of TAK1 and PLCβ2. e Precipitation of endogenous PLCβ2 from RAW264.7 cells expressing GST or GST-TAK1. f Endogenous interaction of PLCβ2 with TAK1 in poly(I:C)-treated primary macrophages. g Confocal analysis of colocolization of TAK1 and PLCβ2 in peritoneal macrophages stimulated with poly(I:C) for 4 h. Scale bar, 5 μm. h IB or IP of lysates from HEK293T cells transfected with FLAG-tagged PLCβ1-3 and HA-tagged TAK1. Data are representative of three experiments with at least three independent biological replicates (n = 3 cultures in f, g)