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. 2019 Feb 14;9:2121. doi: 10.1038/s41598-019-39202-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The detection of LC3-I, LC3-II, and p62 with Western blotting and calculation of LC3-II/LC3-I in cardiomyocytes treated with GA. (a) The bands of LC3-I and LC3-II were analyzed with Western blotting. The positions of LC3-I and LC3-II are indicated by I and II. Western blotting was performed for three independent experiments. β-actin was used as a loading control. Full-length blots are shown in Supplementary Fig. S1. (b) LC3-II/LC3-I was calculated with the band levels of LC3-I and LC3-II. Data are shown as means ± S.D. (N = 3). P-values were based on Tukey’s test. *p < 0.05 vs. the control at each time point. (c) The bands of p62 were analyzed with Western blotting. Western blotting was performed in three independent experiments. β-actin was used as the loading control. Full-length blots are shown in Supplementary Fig. S2.