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. 2019 Feb 14;9:2065. doi: 10.1038/s41598-019-38559-x

Figure 2.

Figure 2

Higher DCV RNA levels in Sgroppino mutants. (A,C) Viral titers in wild-type and Sgp mutant flies inoculated with (A) DCV and (C) CrPV at 24 and 48 hpi. The dashed line represents the detection limit of the titration. (B,D–G) Viral RNA or DNA levels measured by (RT-)qPCR in wild-type and Sgp mutant flies infected with (B) DCV, (D) CrPV, (E) FHV, (F) DXV and (G) IIV-6. Viral RNA/DNA levels were normalized against transcript levels of the housekeeping gene Ribosomal Protein 49 and presented as fold change relative to wild-type flies at 24 hpi (B,D–F) or 3 days post-infection (G). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). Data represent (A,C) mean and s.d. of three independent experiments, each consisting of three replicates of at least 5 female flies for each genotype, or (B,D–G) means and s.d. of three biological replicates of at least 15 female flies for each genotype.