Figure 6.

Peroxisomes are required for host defense to DCV infection. (A) Survival upon DCV infection of flies expressing an RNAi-inducing hairpin targeting Pex3 or GFP. The ubiquitous armadillo driver (arm-Gal4) was used to drive expression of the transcription factor Gal4, which binds to the Upstream Activating Sequence to induce expression a short hairpin RNA targeting Pex3 (Arm > Pex3^RNAi) or GFP (Arm > GFP^RNAi). (B) Pex3 expression levels and (C) viral RNA levels upon DCV infection of Pex3^RNAi flies. Expression of Pex3 was normalized to transcript levels of the housekeeping gene Ribosomal Protein 49 and expressed as percentage of Arm >+ controls. Expression of viral RNA levels was normalized to the housekeeping gene Ribosomal Protein 49 and expressed as fold change relative to the GFP^RNAi control flies at 24 hpi. Data in panels A–C were collected in parallel; knock-down efficiencies in (B) thus apply to the experiments in (A,C). Data represent means and s.d. of three independent pools of (A,C) 5 or (B) 15 female flies for each genotype. Student’s t-tests were used to compare the differences in Pex3 or DCV RNA levels (*P < 0.05, **P < 0.01).