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. 2019 Feb 14;9:2008. doi: 10.1038/s41598-018-37891-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Generation of inhibitory product after 1 h of applying current. (a) Petri dishes containing TSA medium with Ag electrodes and without bacteria were treated with DC for 1 h. (b) Current was switched off and the agar spread with PA. (c) After 24 h, a clear zone of inhibition around the anode was evident. (B) Killing of lawn biofilms of PA grown on TSA after applying current for 1 h (vertical dashed line). The zone of killing continued to extend beyond the width of the electrodes (indicated by horizontal dashed line) upto 27 h after turning the current off. Vertical dotted line shows the time t = 1 h when current was turned off. Curve fitting was done using the online freeware, MyCurveFit.