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. 2019 Feb 14;9:2063. doi: 10.1038/s41598-018-38010-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal microscopy images of hippocampal corpora amylacea (CA) from human brain immunostained with IgMs. (a,b) 60 μm vibratome sections simultaneously immunostained with mouse IgMs against neo-epitopes (green) and with anti-neurofilament heavy (NEFH) (red). DAPI (blue) was used for nuclear staining. Of note, IgM-related immunolabeling is limited to the outer surface. (c,d) Semi-thin 500 nm sections simultaneously immunostained with mouse IgMs against neo-epitopes (green) and with anti-glial fibrillary acidic protein (GFAP) (red). These sections are not stained with DAPI. In semi-thin sections, but not in vibratome sections, CA become sliced up and IgMs can reach the center of the structure. These results indicate that neo-epitopes are present in the whole CA. CA appear to be ensheathed in astrocytic GFAP-filaments (arrowheads), but not stained with NEFH. Scale bar: 10 µm.