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. 2019 Feb 14;9:2096. doi: 10.1038/s41598-018-38312-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SubAB protects against LPS-induced lung inflammation and injury. Wild type C57BL/6 mice were injected i.p. with SubAB or mutant SubA_A272B (0.04 mg/kg bodyweight) for 6 h. Mice were then aerosolized with 6 ml of saline alone or saline containing E.Coli LPS (0.5 mg/ml) for 30 min. Eighteen hours after LPS challenge, lung homogenates were analyzed for levels of BiP/GRP78 (A), proinflammatory mediators VCAM-1 (B) and IL-1β (C) by ELISA and neutrophil sequestration by measuring tissue MPO activity (D). Bronchoalveolar lavage (BAL) fluids were analyzed for albumin levels (E) Lungs were analyzed for wet-to-dry weight ratio (F). Live ventilated mice were evaluated for dynamic lung compliance (G) using whole-body plethysmograph as described in Materials and Methods section.