Figure 1.

Diagram depicting the workflow of preparation, culturing and analysis of precision-cut tissue slices. Inset: A technical drawing (not to scale) showing the dimensions of different components of organotypic slice culture. In the adapted method, the membrane of the insert raised the deep aspect of the tissue slices approximately 1.14 mm from the bottom of the culture well. The tissue slices were submerged in medium (height ~1.31 mm), leaving ~1.0 mm of space for medium to cover the slices, thereby keeping the diffusion distance at a minimum. The space between the insert and the culture dish provided a surface area of ~255 mm², allowing direct gas exchange between the ambient milieu and the culture medium.