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. 2019 Feb 14;9:2064. doi: 10.1038/s41598-018-37950-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of IFX on determination of ATI concentration. Aliquots of human serum from healthy volunteers were spiked with commercial ATI, to the final concentrations indicated, and with IFX (CT-P13, final concentration 8 μg/mL) or its vehicle. Serum aliquots were diluted 30-fold, subjected to acidic pretreatment, and injected over immobilised IFX and IgG (the latter for evaluation of non-specific binding). The points indicate the specific SPR signal due to the binding of flowing ATI to immobilised IFX.