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. 2018 Oct 20;31(2):101–116. doi: 10.1093/intimm/dxy070

Fig. 1.

Fig. 1.

Gating strategy and CD4+ T-cell subset frequencies in TI-LPMC isolated from Ty21a-vaccinated and non-vaccinated volunteers. (A) Freshly isolated TI-LPMC and blood (PBMC) obtained from a Ty21a-vaccinated individual were characterized for memory CD4+ T (CD4+ TM) cell subsets using CD62L and CD45RA markers on gated CD4+ T cells as shown. (B) Frequencies of CD4+ T cells were measured and compared between TI-LPMC obtained from Ty21a-vaccinated (n = 16; red symbols) and unvaccinated volunteers (n = 30; black symbols). (C) Comparison of the frequencies of CD4+ TM subsets [TCM (CD62L+CD45RA−), TEM (CD62L−CD45RA−), TEMRA (CD62L−CD45RA+) and Tnaive (CD62L+CD45RA+)] in TI-LPMC obtained from Ty21a-vaccinated (n = 16; red symbols) and unvaccinated (n = 30; black symbols) volunteers. (D) The frequency of CD4+ TM subsets (TEM, TCM and TEMRA) obtained from TI-LPMC (top panel) and PBMC (bottom panel) co-expressing the activation marker CD69 was determined using flow cytometry. The percentages of CD69 expression are denoted above the corresponding gating boxes in each cytogram. Significant differences between Ty21a-vaccinated and unvaccinated volunteers are denoted as *P < 0.05. Median values for each group are represented as horizontal black bars.