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. 2019 Feb 8;10:91. doi: 10.3389/fpls.2019.00091

FIGURE 3.

FIGURE 3

Nicotiana benthamiana transiently expressing BnaMPK3 enhances resistance to S. sclerotiorum. (A) Diagram of the plasmids of 1300-35S-BnaMPK3-Nos used in this analysis. CaMV35S, cauliflower mosaic virus 35S promoter; NOS, terminator. (B) Expression of BnaMPK3 in N. benthamiana enhanced resistance to S. sclerotiorum. (a) Both left and right panel of the leaves were treated with 1300-35S-BnaMPK3-Nos vector solution. (b) The left panel of a leaves was treated with mock solution, and the right panel of the leaf was treated with 1300-35S-BnaMPK3-Nos vector solution. (c) Both left and right panel of the leaves were treated with mock solution. (d) Both left and right panel of the leaves were treated with 1300-35S-BnaMPK3-Nos vector solution. (C) Relative expression of BnaMPK3 in the treatment with mock or 1300-35S-BnaMPK3-Nos vector. (D) Lesion diameters were measured 36 h post-inoculation. Means and standard errors are shown. Letters indicate statistically significant differences (P < 0.05). The experiment was repeated three times with similar results.