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. 2019 Feb 8;10:91. doi: 10.3389/fpls.2019.00091

FIGURE 6.

FIGURE 6

BnaMPK3 differentially regulates the expression of SA, JA, and ET biosynthesis genes in response to S. sclerotiorum infection. Changes in expression of the genes involving in SA, JA, and ET biosynthesis in the BnaMPK3-overexpressing transgenic line 2 (OE-2), the untransformed wild-type (WT) control and the BnaMPK3-RNA-interfering transgenic line 8 (RNAi-8) plants after 24 hpi. Samples were collected at the indicated time for total RNAs isolation. Expressions of these genes were quantified by real-time PCR, and then change of gene expression (folds of change relative to the level before inoculation) was calculated. Values are means of three replicates, and error bars indicate standard deviations. The significances of the gene expression differences between each transgenic line and WT are indicated (Student’s t-test, ∗∗∗P < 0.001, ∗∗0.001 < P < 0.01 or ∗0.01 < P < 0.05). These experiments were repeated with the BnaMPK3-overexpressing lines 6 and 8, and the BnaMPK3-RNAi lines 16 and 19, and results are similar.