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. 2019 Feb 14;9:2072. doi: 10.1038/s41598-018-37430-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of LHX2 in iPS-HSCs facilitates hepatic maturation of iPS-HPCs. (a) Upper panel: schema for 2D direct co-culture of tdTOMATO⁺ iPS-HPCs (red) and iLHX2-HSCs (green) using the Cytoselect system. Middle panel: representative images of co-cultured cells. Scale bars: 500 μm. Lower panel: representative density plots from flow cytometric analysis of co-cultured cells with vehicle (Veh) or Dox. Gated fractions of co-cultures cells were sorted and analyzed. (b) Expression of α-fetoprotein (AFP), ALB, cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), and apolipoprotein B (APOB) in iPS-HPCs. The y-axis represents the ratio of expression relative to the mean of iPS-HPCs cultured without iPS-HSCs and Dox. (c) Enzyme-linked immunosorbent assay (ELISA) of ALB. ALB production in iPS-HPCs was significantly increased by overexpression of LHX2 in iPS-HSCs. Results represent the mean ± SD of three separate experiments. *P < 0.05.