Table 3.
Primers used for quantitative real‐time PCR
| Gene | Sequence (5′ → 3′) |
|---|---|
| GAPDH | F: AACGGGAAGCTCACTGGCAT |
| R: CTTGACAAAGTGGTCATTGAGGG | |
| CD34 | F: AGTCTGAGGTGAGGCCTCACT |
| R: TGCGGCGGTTCATCAGGAAGT | |
| VEGF‐A | F: CCCACTGAGGAGTTCCAACATCAC |
| R: GGGTTTATACCGGGATTTCTTG | |
| VEGFR‐1 | F: GATGCACAGTGAAATACCCGAAA |
| R: CAGGTTATTCGCTTCCCATCA | |
| VEGFR‐2 | F: TAGTAGGCACGGCAGTGATTG |
| R: GTCGATTCCAAAGGCATCTGC | |
| Ang‐1 | F: AAAGTGTCACACTGGGACAG |
| R: CAGCAGTGTAGAACATTCCA | |
| Ang‐2 | F: TAAAGGACTTACAGGGACGG |
| R: GATCATCATGGTTGTGCCCT | |
| Tie1 | F: CTTGTGAGAACCGAGGTTAC |
| R: GTCTCTGTGGATGAACTGCT | |
| Tie2 | F: AGCTTCTCCATTTCGCAGCGG |
| R: ACTCGATTGCCATCCAGCGCAC |
F, Forward primer.
R, Reverse primer.