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. 2019 Jan 1;9(2):477–490. doi: 10.7150/thno.29361

Figure 5.

Figure 5

Argon enhanced human keratinocyte proliferation but had no significant effect on filaggrin expression in vivo. (a) Keratinocytes were incubated in EpiLife media and exposed to room air (control), nitrogen gas (20% O2 + 5% CO2 + 75% N2) or argon gas (20% O2 + 5% CO2 + 75% Ar). Left: Ki-67 expression (green) in keratinocytes was detected by immunofluorescence staining at 24 hours after gas exposure. Cells were counterstained with DAPI (blue). Right: the number of Ki-67+ cells per 100x magnification field in 10 fields was counted and percentage of Ki-67+ cells was determined (n=8). (b) Mice were exposed to nitrogen (25% O2 + 75% N2) or argon gas (25% O2 + 75% Ar). Left: filaggrin expression in mouse cutaneous wounds was detected by immunohistochemistry. Representative images at 8, 11 and 14 days after injury are shown. Right: fluorescent intensity of filaggrin was quantified by densitometry and normalized to N2 at day 8 (n=10). Data are means ± S.D. ***P<0.001.