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. 2019 Jan 1;9(2):337–354. doi: 10.7150/thno.29026

Figure 3.

Figure 3

The anti-metastatic effects and mechanisms of LMWH and LMWH/PPD. (A) Images and wound healing rates after incubation with HEPES, free LMWH, PPD, free DOX or LMWH/PPD for 24 h (n = 3, means ± SD). *p < 0.05, **p < 0.01. Images were captured at 0 h and 24 h. (B) Images and quantitative analysis of invaded B16F10 cells after incubation with HEPES, free LMWH, PPD, free DOX, LMWH/PPD for 48 h (n = 3, means ± SD). *p < 0.05, **p < 0.01. (C) Actin cytoskeleton of B16F10 cells after incubation with HEPES, free LMWH, PPD, free DOX or LMWH/PPD. The scale bar represents 10 μm. (D) Images of B16F10 cells after incubation with different formulations. (E) Levels of E-cadherin and N-cadherin detected by western blot. a, HEPES; b, HEPES + platelets; c, free Dox + platelets; d, free LMWH + platelets; e, PPD + platelets; f, LMWH/PPD + platelets. Semi-quantitative levels of (F) E-cadherin and (G) N-cadherin normalized to HEPES group (n = 3, means ± SD). *p < 0.05, **p < 0.01, ***p < 0.001.