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. 2019 Feb 8;10:66. doi: 10.3389/fpls.2019.00066

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Copyright © 2019 Yang, Dai, Chen, Yang, Yang, Liu and Jian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spatial and developmental expression of Ha-18764. (A) Localization of Ha18764 mRNA in the dorsal gland of pre-parasitic second stage juveniles of Heterodera avenae by in situ hybridization. The dorsal gland (DG), metacorpus (M), and stylet (S) are indicated with arrows. (B) Developmental expression pattern of Ha18764. The relative expression level of Ha18764 was quantified using qPCR for six different H. avenae stages. The fold-change values were calculated using the 2^-ΔΔCt method and presented as the change in mRNA level in various nematode developmental stages relative to that of egg. Each column represents the mean (±SD) of three samples. This experiment was independently repeated three times, with consistent results. pre-J2, pre-parasitic second-stage juvenile; par-J2, J3, J4, parasitic second-, third- and fourth-stage juveniles, respectively.