FIGURE 8.

Ha18764 suppresses PTI triggered by flg22. (A) Ha18764 suppressed flg22-mediated ROS production in Nicotiana benthamiana. The Agrobacterium tumefaciens strain GV3101 carrying Ha18764, or the empty vector (EV) construct, was infiltrated into N. benthamiana leaves. A total of 18–24 infiltrated leaf disks were collected 48 h post-agroinfiltration and assayed for ROS production. (B) Ha18764 suppressed callose deposition. Ha18764 or Hs18764 transgenic and wild-type (WT, Col-0) Arabidopsis plants were treated with flg22; 24 h later, callose (white dots) in the roots was visualized by staining. Images were photographed in the field of approximately 2 mm² that captures the root area containing the root elongation zone. (C) Quantified relative numbers of callose. (D) Expression of plant defense genes. The mRNA expression levels of WRKY70, WRKY29, PR-1, and CYP81F2 were measured by qPCR in Ha18764 or Hs18764 transgenic and WT plants treated with flg22. Shown are means of three independent biological experiments, each consisting of three technical replicates.