FIGURE 3.

NGO-PEG-PEI/CER induced apoptosis in HCC cells in vitro. (A) Apoptosis was analyzed via Annexin V-FITC/PI double staining. HepG2, HuH7, or PLC/PRF/5 cells were infected with NGO-PEG-PEI/Cer (10 μM), C6-ceramide (10 μM), or NGO-PEG-PEI (100 μg/ml) for 24 h. Florescence was then analyzed by flow cytometry. Data are presented as mean ± SD of three separate experiments. (B) HepG2 cells were infected with NGO-PEG-PEI/Cer (10 μM), C6-ceramide (10 μM), or NGO-PEG-PEI (100 μg/ml) for 24 h. Whole cell extracts were prepared and immunoblotted to detect caspase pathway activation. GAPDH was used as a loading control. ^∗P < 0.05.