Figure 2.

Naive B‐cell‐specific peroxisome proliferator‐activated receptor γ knockout (B‐PPAR‐γ ^−/−) mice have normal splenic CD5⁺ CD1d^hi regulatory B (Breg) and peripheral CD4⁺ CD25⁺ Foxp3⁺ regulatory T (Treg) cells. Splenocytes and peripheral blood were collected from naive control mice and B‐PPAR‐γ ^−/− mice. Representative flow cytometry plots and bar chart show (a) the frequency of CD19⁺ B cells in splenic lymphocytes. Representative flow cytometry plots are gated for lymphocytes. (b) The frequency of CD5⁺ CD1d^hi Breg cells in CD19⁺ B cells and the number of CD5⁺ CD1d^hi Breg cells in spleen. Representative flow cytometry plots are gated for CD19⁺ cells. (c) CD25⁺ Foxp3⁺ Treg cells among the total CD4⁺ T cells in peripheral blood were analyzed. Representative flow cytometry plots are gated for lymphocytes or CD4⁺ cells. Bar graphs indicate mean (± SEM), n = 3, similar results were obtained from two independent experiments.