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. 2019 Jan 29;38(4):e99708. doi: 10.15252/embj.201899708

Figure 1. TRIB1 promotes nuclear accumulation of COP1.

Figure 1

  1. Representative images of COS7 cells expressing GFP‐COP1 (green) co‐transfected with empty vector or the indicated TRIB1‐FLAG construct (red). Cells were stained with anti‐FLAG (Sigma) and anti‐mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm. See also Appendix Fig S1B for Western blots showing the expression levels of the constructs used.
  2. Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP‐COP1 in (A). Mean values ± s.e.m. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP‐COP1 with empty vector was calculated using the Student's t‐test (****P < 0.00001).
  3. Representative images of COS7 cells showing GFP‐COP1 (green) and TRIB1‐FLAG (red) colocalizing in punctate nuclear structures. Insets show enlarged images of these nuclear puncta. Cells were stained with anti‐FLAG (Sigma) and anti‐mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm.
  4. Representative images of COS7 cells showing that GFP‐COP1 (green) and TRIB1‐HA (red) are diffuse in the nucleoplasm when coexpressed with C/EBPα (cyan). Cells were stained with anti‐HA (Santa Cruz), anti‐C/EBPα (Cell Signaling), anti‐mouse Alexa Fluor 405, and anti‐rabbit Alexa Fluor 680. Scale bar, 10 μm. See also Fig EV2C for images of cells expressing each construct alone or in pairs.