Figure EV3. Mutations that disrupt binding to C/EBPα do not affect the ability of TRIB1 to promote nuclear localization of COP1.

• A
  Sequence alignment of the catalytic loop in TRIB1, TRIB2, Drosophila Tribbles (dTrbl), and PKA.
• B–E
  Representative images of COS7 cells showing GFP‐COP1 (green) (B, D) co‐transfected with empty vector or the indicated TRIB1‐FLAG constructs (red). Cells were stained with anti‐FLAG (Sigma) and anti‐mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm. (C, E) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP‐COP1 in (B) and (D). Mean values ± s.e.m. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP‐COP1 coexpressed with TRIB1 WT was calculated using the Student's t‐test (ns, not significant).