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. 2019 Jan 29;38(4):e99708. doi: 10.15252/embj.201899708

Figure EV5. Identification of an intramolecular WD40‐binding site that regulates the subcellular distribution of COP1.

Figure EV5

  1. Schematic of COP1 illustrating locations of the coiled coil–WD40 domain linker deletions and PSL.
  2. Representative images of COS7 cells transfected with the indicated GFP‐COP1 constructs (green). Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm.
  3. Quantification of the average ratio for nuclear/cytoplasmic fluorescence for each of the indicated GFP‐COP1 constructs. Mean values ± s.e.m. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP‐COP1 WT was calculated using the Student's t‐test (****P < 0.00001).
  4. Coimmunoprecipitation of GFP‐COP1 with DET1‐3xFLAG in COS7 cells showing that the 4A mutation weakens the DET1/COP1 interaction.