Figure 2.

KP35 promotes production of the IL-10RB–related cytokines IFN-λ and IL-22. (A) Heatmaps of the IL-10RB signaling pathway recovered from BAL fluid of KP35-infected mice at 48 hours of infection (same samples as in Figures 1A and 1B; n = 3). (B) Immunoblot with densitometry and qRT-PCR of IL-10RB using lung homogenates of WT mice infected with KP35 at the indicated time points. (C) Gene expression measured via qRT-PCR of lung homogenates for IFN-λ (Ifnl2/3) (n = 6) and IL-22 (Il22) (n = 6), and (D) their respective protein levels measured via ELISA (n = 3) or multiplex array (n = 6) in BAL fluid from mice infected with KP35 at the indicated time points. For immunoblots, densitometry values are normalized to actin (n = 3). Representative data from at least two independent experiments are shown. For all graphs, each column is the mean value ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, by one-way ANOVA; Dunnett’s correction for multiple comparisons between groups indicated by the horizontal bar (B–D).