Figure 4.

Ifnlr1^−/− mice are protected from bacteremia. WT and Ifnlr1^−/− mice were infected with KP35 for 48 or 96 hours and compared with PBS-treated controls. (A) Bacterial load was enumerated in BAL fluid (BALF), lung, and spleen. (B) Cell populations in BALF: alveolar macrophages (AMs) (CD45⁺SiglecF⁺CDll11b^lo-mid), granulocytic myeloid-derived suppressor cells/neutrophils (G-MDSCs/NEUTs) (CD45⁺CD11b⁺MHCII^loLy6C^hiLy6G^hi), and monocytic myeloid-derived suppressor cells (M-MDSCs) (CD45⁺CD11b⁺MHCII^loLy6C^hiLy6G^lo). (C) Cytokines in BALF (n = 8–9). (D) Representative immunoblot of lung homogenate for Ocln and Ezr; densitometry is the result of two experiments compiled (n = 4). For the scatter plots, each point represents an individual mouse and horizontal lines within represent median values. ^#Represents the limit of detection. For column graphs, the bar is the mean value ± SEM. Compiled data from at least two independent experiments are shown. Horizontal bars above the data sets equal *P < 0.05, **P < 0.01, Kruskal-Wallis test, one-way ANOVA, Dunn’s correction for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. un = untreated.