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. 2019 Jan 7;38(4):e100303. doi: 10.15252/embj.2018100303

Figure 2. PI4Kβ1 localization at the cell plate and the TGN during cytokinesis in Arabidopsis root cells.

Figure 2

  1. In vivo localization of mCherry‐PI4Kβ1 expressed from the pPI4Kβ1 promoter in root tips of 5‐day‐old complemented pi4kβ1 pi4kβ2 plants. The mCherry‐PI4Kβ1 distribution was imaged using a Zeiss LSM880 in Airyscan Virtual Pinhole (VP) mode with the pinhole set to 2. Arrowheads, nascent cell plates decorated by mCherry‐PI4Kβ1. Scale bar, 20 μm.
  2. Whole‐mount immunostaining of 5‐day‐old seedlings expressing mCherry‐PI4kβ1 in the pi4kβ1 pi4kβ2 double mutant background using anti‐tubulin (red) and anti‐mCherry (green) antibodies, and DAPI (blue). (I), (II), magnifications of regions marked in b, representing early and late cytokinetic stages. Scale bars, 20 μm.
  3. Relative localization of mCherry‐PI4Kβ1 and the TGN marker, ARF1, during cytokinesis. Four‐day‐old seedlings were immunostained with anti‐mCherry (green) and anti‐ARF1 (red) antibodies. Scale bars are 10 μm. Further controls for the use of the anti‐mCherry antibodies can be seen in Appendix Fig S3.