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. 2019 Jan 25;9(3):853–867. doi: 10.7150/thno.29553

Figure 3.

Figure 3

CXCL1 antibody binding specificity and affinity. A, CXCL1 antibody specifically recognizes human CXCL1, but not mouse and rat. B, CXCL1 antibody specifically recognizes CXCL1, but not CXCL2, CXCL3 and CXCL8. C, Specificity of CXCL1 Antibody. The following precipitate samples (Lane 1 - recombinant CXCL1, Lane 2 - serum precipitated with IgG, Lane 3 - 100 ng CXCL1 spiked serum precipitated with IgG, Lane 4 - serum precipitated with HL2401, Lane 5 - 100 ng CXCL1 spiked serum precipitated with HL2401, Lane 6 - 250 ng CXCL1 spiked serum precipitated with HL2401 and Lane 7 - 500 ng CXCL1 spiked serum precipitated with HL2401) were run on a NuPAGE Novex 4-12% Bis-Tris Gel and then stained with the SilverQuest Kit. Arrows indicate CXCL1. C, saturation curve of CXCL1 antibody binding to CXCL1 in PBS and serum by ELISA shows KD of 175.6 ± 30.0 ng/mL in PBS, 386.6 ± 75.4 ng/mL in serum (1:4 dilution), and 252.2 ± 37.1 ng/mL in serum (1:40 dilution).