Figure 4.

CB⁷ reduces the accumulation of PQ in intestine and protects the intestine from injury. (A-C) The accumulation of PQ in the duodenum, jejunum and ileum 4 and 8 h after PQ or PQ@CB⁷ (molar ratio of PQ/CB⁷ = 1:2, CB⁷ = 2.71 g/kg) ingestion at the PQ dose of 300 mg/kg. Data are presented as means ± SEM, n = 6, and *P < 0.05, **P < 0.01. (D-H) Typical pro-inflammatory cytokines (D-F) and ROS related levels (G and H) in jejunum determined at 8 h after mice had ingested saline (control group), CB⁷ (2.71 g/kg), PQ or PQ@CB⁷ (molar ratio of PQ/CB⁷ = 1:2, CB⁷ = 2.71 g/kg) at the PQ dose of 300 mg/kg. Data are presented as means ± SEM, n = 6, and *P < 0.05, **P < 0.01, ***P < 0.001. (I) H&E-stained sections of duodenum collected from the mice sacrificed at 8 h after oral administration with PQ or PQ@CB⁷ (molar ratio of PQ/CB⁷ = 1:2, CB⁷ = 2.71 g/kg) at the PQ dose of 300 mg/kg. The arrows show inflammatory cells infiltration and the triangular arrow showed regions exhibiting epithelial erosion. Scale bar = 200 μm.