Figure 1.

ANTXR2 level was overexpressed in the endometriotic specimens. (A) Genes upregulated in ectopic endometriotic cells (p <0.05 and fold change > 1.5 fold) derived from public datasets (GSE7305 and GSE5108) were cross-referenced with a membrane receptor gene list from GO annotation. (B) Digital analysis of levels of ANTXR2 in the normal and endometriotic specimens. Raw data were retrieved from public datasets (GSE7305 and GSE5108) and analyzed by bioinformatic tools. Endo: ectopic endometriotic tissues collected from women with endometriosis. Nor: endometrial tissues collected from women without endometriosis (defined as normal); (C) ANTXR2 mRNA and (D) protein expression in our own paired samples of endometriosis (n = 12). (E) Representative Western blots (upper panel) and quantified results (lower panel) of ANTXR2 levels in paired primary stromal cells isolated from the same individuals (n = 8). (F, G) Immunohistochemistry (IHC) staining of ANTXR2 was performed by using endometrial (n = 43) and endometriotic specimens (n = 42). ANTXR2 staining was quantified as H score. Eu: eutopic stromal cells; Ec: ectopic stromal cells. St: stromal cell; Epi: epithelial cell. The asterisk (*) indicates P < 0.05 using two-tailed Student's t-test. Results were presented as means ±SEM.