Figure 5.
Knock-out of VEGFC in tumor cells has opposite effects on the formation of tumors in immuno-deficient or immuno-competent mice. (A) Experimental tumors in nude mice (20 mice per condition) were obtained after subcutaneous injection of 5×106 wild-type (WT) or VEGFC-knock-out 786-O cells. Two independent VEGFC-/- clones were injected (VEGFC-/- Cl1 and Cl2). Tumor incidence (percentage of mice with tumors) is presented. (B) Evaluation of tumor size for the experiment described in (A). (C) Experimental tumors in immuno-competent Balb-C mice (10 mice per condition) were obtained after subcutaneous injection of 105 wild-type (WT), heterozygous (vegf -/+) or knock-out RENCA cells (vegfc -/-). The tumor incidence (percentage of mice with a tumor) is shown. (D) Evaluation of the tumor size for the experiment described in (C). When indicated, results are represented as the mean ± SEM. ** p<0.01, *** p<0.001.