Skip to main content
. 2019 Jan 25;9(3):837–852. doi: 10.7150/thno.30228

Figure 3.

Figure 3

Anti-cancer effects of Bcl-2 and PKC-ι siRNA transfected with aptamo- or immune-QLs. The mRNA and protein levels of Bcl-2 and PKC-ι in the transfected MDA-MB-231 cells were determined by RT-PCR (A) and western blotting (B). The viability of the transfected MDA-MB-231 cells (200 nM siRNA each) was measured by the CCK-8 assay after 48 h incubation (C). Each bar represents the mean ± S.D. from 5 separate experiments. ***p < 0.001 vs. control. (D) Invasion of MDA-MB-231 cells transfected with PKC-ι siRNA was measured by counting the invaded cells through membranes per field. *p < 0.05 vs. control. Migration of the transfected cells was also compared with that of control cells by examining the wound healing with a microscope (E) and analyzing the percentage of wound closure with Image J software (F). Each bar represents the mean ± S.D. from 3 separate experiments. ***p < 0.001 vs. 0 h.